KANK2 Polyclonal antibody

KANK2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20546-1-AP

Synonyms

MXRA3, Matrix-remodeling-associated protein 3, KN motif and ankyrin repeat domain-containing protein 2, KIAA1518, Ankyrin repeat domain-containing protein 25

---

Validation Data Gallery

• 

  IHC staining of human colon using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse ovary using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20546-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KANK2 antibody (20546-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive IHC detected in: human colon tissue, human kidney tissue, mouse heart tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20546-1-AP targets KANK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KN motif and ankyrin repeat domains 2
• Calculated Molecular Weight: 91 kDa
• GenBank Accession Number: NM_001136191
• Gene Symbol: KANK2
• Gene ID (NCBI): 25959
• RRID: AB_10694286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.

Publications

Species	Application	Title
human	IHC	Lab Med; Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer; Authors - Shiquan Xu