JUNB Polyclonal antibody

• Featured Product
• KD/KO Validated

JUNB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10486-1-AP

Synonyms

AP-1, AP 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10486-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10486-1-AP

  IP result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lymphoma using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 10486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using JUNB antibody (10486-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10486-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (10486-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF7 cells, C6 cells, HeLa cells, HT-1080 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue, human breast cancer tissue, human ovary tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

10486-1-AP targets JUNB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JUNB fusion protein Ag0752
• Full Name: jun B proto-oncogene
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 42 kDa, 43 kDa
• GenBank Accession Number: BC004250
• Gene Symbol: JUNB
• Gene ID (NCBI): 3726
• RRID: AB_2129996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	WB	J Proteome Res; Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.; Authors - Bing Wang
human	WB	Exp Cell Res; An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1; Authors - Xuanxuan Hu