JTV1 Polyclonal antibody

• Featured Product
• KD/KO Validated

JTV1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 10424-1-AP

Synonyms

Protein JTV-1, PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

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Validation Data Gallery

• 

  WB analysis of HeLa using 10424-1-AP

  WB result of JTV1 antibody (10424-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-JTV1 transfected HeLa cells.

• 

  WB analysis using 10424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10424-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10424-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10424-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10424-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10424-1-AP

  IP result of anti-JTV1 (IP:10424-1-AP, 3ug; Detection:10424-1-AP 1:1000) with HeLa cells lysate 800ug.

• 

  IHC staining of mouse cerebellum using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10424-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JTV1 antibody (10424-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HepG2 using 10424-1-AP

  Immunofluorescent analysis of HepG2 cells, using JTV1 antibody 10424-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, rat liver tissue, L02 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse cerebellum tissue, human kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Product Information

10424-1-AP targets JTV1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JTV1 fusion protein Ag0675
• Full Name: JTV1 gene
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002853
• Gene Symbol: JTV1
• Gene ID (NCBI): 7965
• RRID: AB_513880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1. There're two isoforms of JTV1 with MW about 27 kDa and 35 kDa,

Publications

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
mouse	WB,IF	Nat Neurosci; Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss.; Authors - Lee Yunjong Y
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao
human	WB,IF	Nat Commun; Molecular determinants of selective clearance of protein inclusions by autophagy.; Authors - Wong Esther E
mouse	WB	Proc Natl Acad Sci U S A; Role for the obesity-related FTO gene in the cellular sensing of amino acids.; Authors - Gulati Pawan P
rat	WB	Proc Natl Acad Sci U S A; Phosphorylation by the c-Abl protein tyrosine kinase inhibits parkin's ubiquitination and protective function.; Authors - Ko Han Seok HS

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

KRishnendu (Verified Customer) (02-27-2020)

Very strong antibody with no contaminant bands.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB; 1:100 for IP
• Cell Tissue Type: HEK293T

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