Filter: AllWBIHCIF/ICC

WB analysis using 51151-1-AP (same clone as 51151-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
WB analysis using 51151-1-AP (same clone as 51151-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 51151-1-AP (same clone as 51151-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 51151-1-AP (same clone as 51151-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 51151-1-AP (same clone as 51151-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)
Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 51151-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

51151-1-PBS targets JNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	44-48 kDa, 50-55 kDa
GenBank Accession Number	NM_138982
Gene Symbol	JNK
Gene ID (NCBI)	5599
RRID	AB_2140710
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P45983
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.