JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10023-1-PBS

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10023-1-AP (same clone as 10023-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10023-1-AP (same clone as 10023-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10023-1-AP (same clone as 10023-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10023-1-AP (same clone as 10023-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10023-1-AP (same clone as 10023-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW264.7 using 10023-1-AP (same clone as 10023-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 10023-1-AP (same clone as 10023-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10023-1-AP (same clone as 10023-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10023-1-AP (same clone as 10023-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10023-1-AP (same clone as 10023-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10023-1-AP (same clone as 10023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10023-1-AP (same clone as 10023-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (10023-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

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Product Information

10023-1-PBS targets JNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag0019
• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 40-45 kDa, 50-55 kDa
• GenBank Accession Number: NM_138982
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.