Jurkat cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25098-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 25098-1-AP
IP result of anti-JMY (IP:25098-1-AP, 4ug; Detection:25098-1-AP 1:600) with Jurkat cells lysate 2400ug.
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 25098-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JMY antibody (25098-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JMY antibody (25098-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Junction mediating and regulatory protein(JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.
Jurkat cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25098-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25098-1-AP (JMY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 25098-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25098-1-AP (JMY Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of Jurkat using 25098-1-AP
IP result of anti-JMY (IP:25098-1-AP, 4ug; Detection:25098-1-AP 1:600) with Jurkat cells lysate 2400ug.
IF/ICC Figures
IF Staining of HeLa using 25098-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JMY antibody (25098-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
JMY Polyclonal antibody
Catalog Number
25098-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:2000-1:8000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
