JMJD5 Polyclonal antibody

JMJD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20999-1-AP

Synonyms

JMJD5, jumonji domain containing 5, KDM8, Lysine specific demethylase 8

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Validation Data Gallery

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  WB analysis using 20999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20999-1-AP (JMJD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-453s cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20999-1-AP targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JMJD5 fusion protein Ag15115
• Full Name: jumonji domain containing 5
• Calculated Molecular Weight: 416 aa, 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC027911
• Gene Symbol: JMJD5
• Gene ID (NCBI): 79831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JmjC (Jumonji-C) domain-containing 5 (JMJD5) (also called KDM8) is a 2-oxoglutarate (2-OG) dependent oxygenase of the JmjC subfamily. By shuttling between the nucleus and cytoplasm, JMJD5 could either associate with histones or cytosolic proteins, and thus plays an important role in embryonic development (PMID:22241836;22402282), circadian regulation (PMID:21115819), osteoclastogenesis (PMID:22375008), and stem-cell renewal (PMID:24740926).