FcZero-rAb® APC Anti-Human JAM-A/CD321 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using APC-FcA98328-3

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human JAM-A/CD321 Rabbit RecAb (APC-FcA98328-3, Clone: 242290B3) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using APC-FcA98328-3

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human JAM-A/CD321 Rabbit RecAb (APC-FcA98328-3, Clone: 242290B3) (red) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

JAM-A/CD321 FcZero-rAb^® Recombinant Antibody for FC

Cat No. APC-FcA98328-3

Clone No.242290B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, CD321, F11 receptor, JAM

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98328-3 targets JAM-A/CD321 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1372

  Product name: Recombinant Human JAM-A/Junctional Adhesion Molecule A protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 28-238 aa of NM_016946.6

  Sequence: SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

• Full Name: F11 receptor
• Calculated Molecular Weight: 33kDa
• GenBank Accession Number: NM_016946.6
• Gene Symbol: F11R
• Gene ID (NCBI): 50848
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y624-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.