JAKMIP1 Polyclonal antibody

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  WB analysis of mouse spleen using 13846-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13846-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13846-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13846-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13846-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13846-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13846-1-AP

  IP result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

JAKMIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13846-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MARLIN1, Janus kinase and microtubule-interacting protein 1, janus kinase and microtubule interacting protein 1, JAMIP1, GABABRBP

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13846-1-AP targets JAKMIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JAKMIP1 fusion protein Ag4816
• Full Name: janus kinase and microtubule interacting protein 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC047075
• Gene Symbol: JAKMIP1
• Gene ID (NCBI): 152789
• RRID: AB_2128793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96N16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Neuron; JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.; Authors - Jamee M Berg ; KO Validated
human	WB,IHC	Biomed Pharmacother; Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.; Authors - Okai Isaac I