JAK3 Recombinant antibody

JAK3 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3N1

Cat no : 80331-1-RR

Synonyms

JAK 3, JAK3, JAK3_HUMAN, JAKL, Janus kinase 3, L JAK, Leukocyte janus kinase, LJAK, Tyrosine protein kinase JAK3

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Validation Data Gallery

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  WB analysis using 80331-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80331-1-RR (JAK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 80331-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 80331-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

80331-1-RR targets JAK3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Calculated Molecular Weight: 1124 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC028068
• Gene Symbol: JAK3
• Gene ID (NCBI): 3718
• RRID: AB_2918888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)

Publications

Species	Application	Title
human	WB	Front Immunol; Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion; Authors - Pengping Li
human	IHC	Gene; The JAK-STAT signaling-related signature serves as a prognostic and predictive biomarker for renal cell carcinoma immunotherapy; Authors - Szehoi Chan
mouse	IHC	Front Genet; Identification of apoptosis-immune-related gene signature and construction of diagnostic model for sepsis based on single-cell sequencing and bulk transcriptome analysis; Authors - Zhongyi Sun
human	IHC	Clin Exp Rheumatol; Effectiveness of tofacitinib monotherapy for patients with IgG4-RD or idiopathic retroperitoneal fibrosis; Authors - Xiaoyu Cao