Jagged 2 Polyclonal antibody

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• KD/KO Validated

Jagged 2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19696-1-AP

Synonyms

HJ2, JAG2, jagged 2, Jagged2, Protein jagged 2, SER2

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Validation Data Gallery

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  WB analysis of HeLa using 19696-1-AP

  WB result of Jagged 2 antibody (19696-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Jagged 2 transfected HeLa cells.

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  WB analysis using 19696-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19696-1-AP (Jagged 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19696-1-AP targets Jagged 2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: jagged 2
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_002226
• Gene Symbol: Jagged 2
• Gene ID (NCBI): 3714
• RRID: AB_2878598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.; Authors - Zhikun Zheng
human	WB	Gene; ZEB2 alleviates Hirschsprung's-associated enterocolitis by promoting the proliferation and differentiation of enteric neural precursor cells via the Notch-1/Jagged-2 pathway; Authors - Yong Feng