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Islet 1 Recombinant antibody, PBS Only (Detector)

Islet 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240833D7

Cat no : 83784-4-PBS

Synonyms

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Islet 1 Recombinant antibody (83784-4-RR)

    WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Islet 1 Recombinant antibody (83784-4-RR)

    IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00771-3

    Cytometric bead array standard curve of MP00771-3

    Cytometric bead array standard curve of MP00771-3, Islet 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83784-2-PBS. Detection antibody: 83784-4-PBS. Standard: Ag33541. Range: 0.313- 40 ng/mL.

Product Information

83784-4-PBS targets Islet 1 as part of a matched antibody pair:

MP00771-3: 83784-2-PBS capture and 83784-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Islet 1 fusion protein Ag33541
Full Name ISL LIM homeobox 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC031213
Gene Symbol Islet 1
Gene ID (NCBI) 3670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.