Involucrin Polyclonal antibody

Involucrin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 28462-1-AP

Synonyms

IVL

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Validation Data Gallery

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  WB analysis of A431 using 28462-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 28462-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28462-1-AP (Involucrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 28462-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 28462-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Involucrin antibody (28462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 28462-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Involucrin Polyclonal antibody (28462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HT-1080 cells, HT-29 cells
• Positive IHC detected in: human tonsillitis tissue, human brown disease, human oesophagus cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

28462-1-AP targets Involucrin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Involucrin fusion protein Ag29585
• Full Name: involucrin
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_005547
• Gene Symbol: Involucrin
• Gene ID (NCBI): 3713
• RRID: AB_2881148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

Publications

Species	Application	Title
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
	IF	Int Immunopharmacol; Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway.; Authors - Jie-Fang Gao
	WB,IHC	J Ethnopharmacol; Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.; Authors - Zhenzhuo Li
human	WB	J Oncol; Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma; Authors - Shiyu Zeng
human	WB	Biol Pharm Bull; Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes; Authors - Yushi Katsuyama
human	WB	Front Pharmacol; Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation; Authors - Lusheng Wang