IFN-beta Polyclonal antibody

IFN Beta Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 27506-1-AP

Synonyms

Fibroblast IFN, IFB, IFF, IFN beta, IFNB, IFNB1, IFN-beta

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Validation Data Gallery

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  WB analysis of Recombinant protein using 27506-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 27506-1-AP (IFN Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using IFN Beta antibody (27506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27506-1-AP (IFN-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IF/ICC detected in: L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

27506-1-AP targets IFN Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFN Beta fusion protein Ag26326
• Full Name: IFN beta
• Calculated Molecular Weight: 187 aa, 22 kDa
• GenBank Accession Number: BC069314
• Gene Symbol: IFN beta1
• Gene ID (NCBI): 3456
• RRID: AB_2880893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	WB	Amino Acids; Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.; Authors - Yan Jin
human	WB	BMC Vet Res; The role of IBV PL1pro in virus replication and suppression of host innate immune responses; Authors - Weirong Liu
human	IF	Cell Death Dis; Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion.; Authors - Tongtian Zhuang
		Virology; Role of heparan sulfate in the Zika virus entry, replication, and cell death.; Authors - Huixin Gao