Integrin beta-6 Polyclonal antibody

Integrin beta-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28378-1-AP

Synonyms

Integrin beta 6, Integrin beta-6, integrin, beta 6, ITGB6

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Validation Data Gallery

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  WB analysis using 28378-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 28378-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 28378-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28378-1-AP (Integrin beta-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

28378-1-AP targets Integrin beta-6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin beta-6 fusion protein Ag29002
• Full Name: integrin, beta 6
• Calculated Molecular Weight: 788 aa, 86 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC121178
• Gene Symbol: Integrin beta 6
• Gene ID (NCBI): 3694
• RRID: AB_2918157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

Publications

Species	Application	Title
human	IHC	Front Oncol; The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma; Authors - Junjian Yu
human	WB,IHC	Transl Lung Cancer Res; Exosomal ITGB6 from dormant lung adenocarcinoma cells activates cancer-associated fibroblasts by KLF10 positive feedback loop and the TGF-β pathway; Authors - Xiang Feng