Integrin beta-5 Recombinant antibody, PBS Only (Capture/Detector)

Integrin beta-5 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240401G11

Cat no : 83514-3-PBS

Synonyms

ITGB5, integrin, beta 5, Integrin beta 5, 240401G11

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Validation Data Gallery

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  WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00512-4

  Sandwich ELISA standard curve of MP00512-4, Integrin beta-5 Recombinant Matched Antibody Pair - PBS only. 83514-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29783.83514-1-PBS was HRP conjugated as the detection antibody. Range: 9.766-625 pg/mL.

• 

  Sample test of MP00512-4

  The mean Integrin beta-5 concentration was determined to be 275.71 pg/mL in Hela cell extract based on a 2.8 mg/mL extract load and 190.06 pg/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load.

• 

  IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00512-1

  Cytometric bead array standard curve of MP00512-1, Integrin beta-5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-2-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

• 

  Cytometric bead array standard curve of MP00512-2

  Cytometric bead array standard curve of MP00512-2, Integrin beta-5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-5-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83514-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

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Product Information

83514-3-PBS targets Integrin beta-5 as part of a matched antibody pair:

MP00512-1: 83514-2-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-2: 83514-5-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-4: 83514-3-PBS capture and 83514-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Integrin beta-5 fusion protein Ag29783
• Full Name: integrin, beta 5
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 88-100 kDa
• GenBank Accession Number: BC006541
• Gene Symbol: Integrin beta 5
• Gene ID (NCBI): 3693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.