Integrin alpha-E Monoclonal antibody, PBS Only

Integrin alpha-E Monoclonal Antibody for IF/ICC, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B6G6

Cat no : 68893-3-PBS

Synonyms

CD103, 4B6G6, Integrin alpha E, HUMINAE, HML-1 antigen

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Validation Data Gallery

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  IF Staining of human PBMCs using 68893-3-Ig (same clone as 68893-3-PBS)

  Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 68893-3-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 68893-3-Ig (same clone as 68893-3-PBS)

  1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed. This data was developed using the same antibody clone with 68893-3-PBS in a different storage buffer formulation.

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Product Information

68893-3-PBS targets Integrin alpha-E in IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin alpha-E fusion protein Ag27410
• Full Name: integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 1179 aa, 130 kDa
• GenBank Accession Number: BC113436
• Gene Symbol: ITGAE
• Gene ID (NCBI): 3682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G Magarose purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)