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Integrin alpha-E Monoclonal antibody

Integrin alpha-E Monoclonal Antibody for IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4B6G6

Cat no : 68893-3-Ig

Synonyms

CD103, 4B6G6, Integrin alpha E, HUMINAE, HML-1 antigen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human PBMCs using Integrin alpha-E Monoclonal antibody (68893-3-Ig)

    IF Staining of human PBMCs using 68893-3-Ig

    Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

  • Flow cytometry (FC) experiment of human PBMCs using Integrin alpha-E Monoclonal antibody (68893-3-Ig)

    FC experiment of human PBMCs using 68893-3-Ig

    1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed.

Tested Applications

Positive IF/ICC detected in5 µg/mL PHA ( 3 days) treated human PBMCs
Positive FC detected in5 µg/mL PHA for 3 days human PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68893-3-Ig targets Integrin alpha-E in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin alpha-E fusion protein Ag27410
Full Name integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
Calculated Molecular Weight 1179 aa, 130 kDa
GenBank Accession NumberBC113436
Gene Symbol ITGAE
Gene ID (NCBI) 3682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G Magarose purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)

Protocols

Product Specific Protocols
IF protocol for Integrin alpha-E antibody 68893-3-IgDownload protocol
FC protocol for Integrin alpha-E antibody 68893-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols