Integrin alpha-8 Polyclonal antibody

Integrin alpha-8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30714-1-AP

Synonyms

Integrin alpha 8, integrin, alpha 8, ITGA8

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Validation Data Gallery

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  WB analysis using 30714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 30714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 30714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 30714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse lung using 30714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue, rat spleen tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30714-1-AP targets Integrin alpha-8 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin alpha-8 fusion protein Ag33507
• Full Name: integrin, alpha 8
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003638
• Gene Symbol: Integrin alpha 8
• Gene ID (NCBI): 8516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).