Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody

Integrin alpha-5/CD49e Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241685D4

Cat no : 98171-1-RR

Synonyms

Integrin Alpha-5, ITGA5, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 98171-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98171-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98171-1-RR targets Integrin alpha-5/CD49e in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).