Integrin alpha-5 Monoclonal antibody, PBS Only

Integrin alpha-5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B9E5

Cat no : 68561-1-PBS

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

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Validation Data Gallery

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  WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 68561-1-Ig (same clone as 68561-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 68561-1-Ig (same clone as 68561-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 68561-1-Ig (same clone as 68561-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68561-1-PBS targets Integrin alpha-5 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin alpha-5 fusion protein Ag33642
• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: ITGA5
• Gene ID (NCBI): 3678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).