Integrin Alpha 4 Monoclonal antibody

Integrin Alpha 4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B6A3

Cat no : 67040-1-Ig

Synonyms

ITGA4, Integrin alpha-4, Integrin alpha IV, IA4, CD49D

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Validation Data Gallery

• 

  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67040-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NK-92 using 67040-1-Ig

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 67040-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MOLT-4 cells, Raji cells, U-937 cells, NK-92 cells, THP-1 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67040-1-Ig targets Integrin Alpha 4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin Alpha 4 fusion protein Ag28024
• Full Name: integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Calculated Molecular Weight: 1032 aa, 115 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC156712
• Gene Symbol: Integrin alpha 4
• Gene ID (NCBI): 3676
• RRID: AB_2882354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Publications

Species	Application	Title
human	WB	Blood Adv; SORT1/LAMP2-mediated Extracellular Vesicle Secretion and Cell Adhesion Are Linked to Lenalidomide Resistance in Multiple Myeloma.; Authors - Tomofumi Yamamoto