Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29042-1-AP

Synonyms

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a

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Validation Data Gallery

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  WB analysis using 29042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 29042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, rat lung tissue
• Positive IHC detected in: human placenta tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29042-1-AP targets Integrin alpha 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin alpha 1 fusion protein Ag30613
• Full Name: integrin, alpha 1
• Calculated Molecular Weight: 1179 aa, 131 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC137121
• Gene Symbol: Integrin alpha 1
• Gene ID (NCBI): 3672
• RRID: AB_2881238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publications

Species	Application	Title
human	WB	Development; TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration; Authors - Binbin Li
human	WB	PeerJ; Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions; Authors - Mengru Bai