Integrin Beta 4 Monoclonal antibody, PBS Only

Integrin Beta 4 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C2A11

Cat no : 66922-2-PBS

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Validation Data Gallery

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  WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 66922-2-Ig (same clone as 66922-2-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 66922-2-Ig (same clone as 66922-2-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66922-2-Ig (same clone as 66922-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin beta-4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66922-2-Ig (same clone as 66922-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66922-2-PBS targets Integrin Beta 4 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin Beta 4 fusion protein Ag17395
• Full Name: integrin, beta 4
• Calculated Molecular Weight: 1752 aa, 195 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC126411
• Gene Symbol: ITGB4
• Gene ID (NCBI): 3691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.