Ins1 Polyclonal antibody

Ins1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28609-1-AP

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I, insulin1

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Validation Data Gallery

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  WB analysis using 28609-1-AP

  Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 28609-1-AP (insulin1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of rat pancreas using 28609-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 28609-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 28609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 28609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat pancreas tissue
• Positive IHC detected in: rat pancreas tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28609-1-AP targets Ins1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ins1 fusion protein Ag29989
• Full Name: insulin I
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_008386
• Gene Symbol: Ins1
• Gene ID (NCBI): 16333
• RRID: AB_2918180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.