IL-6 Polyclonal antibody

IL-6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 26404-1-AP

Synonyms

Il 6, Il6, Il-6, interleukin 6

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Validation Data Gallery

• 

  WB analysis using 26404-1-AP

  untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• ELISA: See 1 publications below

Product Information

26404-1-AP targets IL-6 in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-6 fusion protein Ag24800
• Full Name: interleukin 6
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_031168
• Gene Symbol: Il6
• Gene ID (NCBI): 16193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

Species	Application	Title
mouse	WB,IHC	Sci Total Environ; Neurotoxic effects of triclosan in adolescent mice: Pyruvate kinase M2 dimer regulated signal transducer and activator of transcription 3 phosphorylation mediated microglia activation and neuroinflammation; Authors - Ziyun Wei
mouse	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
	WB	J Transl Med; Immunotherapy landscape analyses of necroptosis characteristics for breast cancer patients.; Authors - Honghao Yu
mouse	WB	BMC Complement Med Ther; Ethanol extract of Paridis rhizoma attenuates carrageenan-induced paw swelling in rats by inhibiting the production of inflammatory factors; Authors - Li Xiang
mouse	WB	World J Diabetes; Depletion of gut microbiota facilitates fibroblast growth factor 21-mediated protection against acute pancreatitis in diabetic mice; Authors - Qi-Yan Sun
	WB	Free Radic Biol Med; Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy; Authors - Jing Ma