IL3RA/CD123 Polyclonal antibody

IL3RA/CD123 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30012-1-AP

Synonyms

Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha

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Validation Data Gallery

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  WB analysis using 30012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 30012-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-12 cells, NIH/3T3 cells
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30012-1-AP targets IL3RA/CD123 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL3RA/CD123 fusion protein Ag32002
• Full Name: interleukin 3 receptor, alpha chain
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa, 60-70 kDa
• GenBank Accession Number: NM_008369
• Gene Symbol: Il3ra
• Gene ID (NCBI): 16188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily (PMID: 25043189). It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors. This antibody is specific for mouse Il3ra.