IL-17a Polyclonal antibody

IL-17a Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 26163-1-AP

Synonyms

Ctla 8, Ctla8, Il17, Il17a, Il-17a, interleukin 17A

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Validation Data Gallery

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  WB analysis using 26163-1-AP

  PMA and ionomycin treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26163-1-AP (IL-17a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells
• Positive IHC detected in: mouse spleen tissue, human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 14 publications below
• IF: See 9 publications below

Product Information

26163-1-AP targets IL-17a in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-17a fusion protein Ag24087
• Full Name: interleukin 17A
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15kDa,17 kDa
• GenBank Accession Number: NM-010552
• Gene Symbol: Il17a
• Gene ID (NCBI): 16171
• RRID: AB_2880409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The IL17A monomer is a peptide consisting of 155 amino acids. The IL17A peptide comprises a 23 amino acid signal peptide and a 132 amino acid mature peptide. The IL17A homodimer has a molecular weight of 35 kD (Kolls and Lindén, 2004).

Publications

Species	Application	Title
mouse	IHC	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Cell Biol Toxicol; Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19.; Authors - Chunfeng Xie
mouse	WB,IHC	Int Immunopharmacol; Pathogenic Th17 cell-mediated liver fibrosis contributes to resistance to PD-L1 antibody immunotherapy in hepatocellular carcinoma; Authors - Meiying Song
human,mouse	IHC	J Cell Mol Med; Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.; Authors - Lihua Gao
mouse	IF	Front Microbiol; Eucommia ulmoides bark extract reduces blood pressure and inflammation by regulating the gut microbiota and enriching the Parabacteroides strain in high-salt diet and N(omega)-nitro-L-arginine methyl ester induced mice; Authors - Dong Yan
mouse	IHC	Biochim Biophys Acta Mol Cell Res; NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis; Authors - Youhua Peng