IL-17a Polyclonal antibody

IL-17a Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26163-1-AP

Synonyms

Ctla 8, Ctla8, Il17, Il17a, Il-17a, interleukin 17A



Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated EL-4 cells
Positive IHC detected inmouse spleen tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26163-1-AP targets IL-17a in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-17a fusion protein Ag24087
Full Name interleukin 17A
Calculated Molecular Weight17 kDa
Observed Molecular Weight15kDa,17 kDa
GenBank Accession NumberNM-010552
Gene Symbol Il17a
Gene ID (NCBI) 16171
RRIDAB_2880409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The IL17A monomer is a peptide consisting of 155 amino acids. The IL17A peptide comprises a 23 amino acid signal peptide and a 132 amino acid mature peptide. The IL17A homodimer has a molecular weight of 35 kD (Kolls and Lindén, 2004).

Protocols

Product Specific Protocols
WB protocol for IL-17a antibody 26163-1-APDownload protocol
IHC protocol for IL-17a antibody 26163-1-APDownload protocol
IF protocol for IL-17a antibody 26163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
humanWB

Cell Biol Toxicol

Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19.

Authors - Chunfeng Xie
mouseWB,IHC

Int Immunopharmacol

Pathogenic Th17 cell-mediated liver fibrosis contributes to resistance to PD-L1 antibody immunotherapy in hepatocellular carcinoma

Authors - Meiying Song
human,mouseIHC

J Cell Mol Med

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.

Authors - Lihua Gao
mouseIF

Front Microbiol

Eucommia ulmoides bark extract reduces blood pressure and inflammation by regulating the gut microbiota and enriching the Parabacteroides strain in high-salt diet and N(omega)-nitro-L-arginine methyl ester induced mice

Authors - Dong Yan
mouseIHC

Biochim Biophys Acta Mol Cell Res

NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis

Authors - Youhua Peng