IkB Alpha Recombinant antibody, PBS Only

IkB Alpha Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C24

Cat no : 80019-1-PBS

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

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Validation Data Gallery

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  WB analysis using 80019-1-RR (same clone as 80019-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80019-1-RR (IkB alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80019-1-RR (same clone as 80019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80019-1-RR (IkB alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80019-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80019-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IkB Alpha fusion protein Ag0345
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa and 35 kDa
• GenBank Accession Number: BC002601
• Gene Symbol: NFKBIA
• Gene ID (NCBI): 4792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. IkB alpha can be observed as a band at 36 kDa in human cell lysates, and 35 kDa in mouse and rat cell ysates.