Phospho-IkB Alpha (Ser32/36) Monoclonal antibody, PBS Only

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  WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

  Untreated PC-3 cells, PC-3 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control. This data was developed using the same antibody clone with 68999-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68999-1-Ig (same clone as 68999-1-PBS)

  Untreated MCF-7 cells, MCF-7 cells (treated with 100 nM Calyculin A for 0.5 hours) were subjected to SDS PAGE followed by western blot with 68999-1-Ig (Phospho-IkB Alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as loading control. This data was developed using the same antibody clone with 68999-1-PBS in a different storage buffer formulation.

Phospho-IkB Alpha (Ser32/36) Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68999-1-PBS

Clone No.2G8E5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IKBA, IkB alpha, IκB α, NFKBIA, I-kappa-B-alpha

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Product Information

68999-1-PBS targets Phospho-IkB Alpha (Ser32/36) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25963
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha).