Mouse IgG2a Isotype Control (MOPC-173)

• 

  FC experiment of human PBMCs using 65244-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG2a Isotype Control (65244-1-Ig, Clone:MOPC-173) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Anti-Human CD3 (Hit3a) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

IgG2a Isotype Control Monoclonal Antibody for FC

Cat No. 65244-1-Ig

Clone No.MOPC-173

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Synonyms

IgG2a, IgG2a Isotype Control

---

Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65244-1-Ig targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_3670281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.