Human IgG Lambda Light Chain Polyclonal antibody

Human IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20758-1-AP

Synonyms

IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC

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Validation Data Gallery

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  WB analysis of human plasma using 20758-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 20758-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20758-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 20758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Lambda Light Chain antibody (20758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

20758-1-AP targets Human IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Human IgG Lambda Light Chain fusion protein Ag14693
• Full Name: immunoglobulin lambda constant 1 (Mcg marker)
• Calculated Molecular Weight: 233 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC007782
• Gene Symbol: IgG Lambda Light Chain
• Gene ID (NCBI): 3537
• RRID: AB_2878734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the lambda light chain of human IgG.

Publications

Species	Application	Title
human,mouse	IHC,IF,FC	iScience; SFTSV infection is associated with transient overproliferation of monoclonal lambda-type plasma cells; Authors - Chuansong Quan