Anti-Mouse IFN-gamma Rabbit Recombinant Antibody, PBS Only

IFN-gamma Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240262C9

Cat no : 98045-1-PBS

Synonyms

IFN gamma, Ifng, IFN γ, IFN g, Ifg

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Validation Data Gallery

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  FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR (same clone as 98045-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98045-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98045-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98045-1-RR against Mouse IFN-gamma were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 98045-1-PBS is IFN-gamma Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interferon gamma
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: BC119063
• Gene Symbol: IFN gamma
• Gene ID (NCBI): 15978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)