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Anti-Mouse IFN-gamma Rabbit Recombinant Antibody

IFN-gamma Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240262C9

Cat no : 98045-1-RR

Synonyms

IFN gamma, Ifng, IFN γ, IFN g, Ifg

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 Th1-polarized splenocytes using Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR)

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with APC Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of C57BL/6 Th1-polarized splenocytes using Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR)

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    1x10^6 PMA, Ionomycin and Brefeldin A treated(red) or untreated(blank) C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98045-1-RR

    Affinity and Kinetic Characterization of 98045-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98045-1-RR against Mouse IFN-gamma were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98045-1-RR is IFN-gamma Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interferon gamma
Calculated Molecular Weight17 kDa
GenBank Accession NumberBC119063
Gene Symbol IFN gamma
Gene ID (NCBI) 15978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protocols

Product Specific Protocols
FC protocol for IFN-gamma antibody 98045-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols