ICAM-1/CD54 Polyclonal antibody

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30324-1-AP

Synonyms

Icam-1, CD54, MALA-2, Icam1, ICAM 1

---

Validation Data Gallery

• 

  WB analysis using 30324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30324-1-AP (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30324-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 30324-1-AP (Icam-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 30324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 30324-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue
• Positive IHC detected in: mouse liver tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30324-1-AP targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1.