IVNS1ABP Recombinant antibody

IVNS1ABP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241587B1

Cat no : 84268-1-RR

Synonyms

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

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Validation Data Gallery

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  WB analysis using 84268-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 84268-1-RR

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84268-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 84268-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84268-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: U-251 cells, mouse ovary tissue, HEK-293 cells, HepG2 cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84268-1-RR is IVNS1ABP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IVNS1ABP fusion protein Ag6412
• Full Name: influenza virus NS1A binding protein
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC067739
• Gene Symbol: IVNS1ABP
• Gene ID (NCBI): 10625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.