IVNS1ABP Polyclonal antibody

IVNS1ABP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14741-1-AP

Synonyms

ARA3, FLARA3, HSPC068, IVNS1ABP, KIAA0850, ND1, NS 1, NS1, NS1 binding protein, NS1 BP, NS1BP

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Validation Data Gallery

• 

  WB analysis of mouse heart using 14741-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 14741-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14741-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14741-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14741-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 14741-1-AP

  IP result of anti-IVNS1ABP (IP:14741-1-AP, 4ug; Detection:14741-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, Apoptosised HeLa cells, HEK-293 cells, HeLa cells, K-562 cells, mouse ovary tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14741-1-AP targets IVNS1ABP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IVNS1ABP fusion protein Ag6412
• Full Name: influenza virus NS1A binding protein
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC067739
• Gene Symbol: IVNS1ABP
• Gene ID (NCBI): 10625
• RRID: AB_10638784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger