ITPK1 Polyclonal antibody

ITPK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10568-1-AP

Synonyms

Ins(1,3,4)P(3) 5/6 kinase, ITPK1, ITRPK1

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Validation Data Gallery

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  WB analysis of human brain using 10568-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10568-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 10568-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10568-1-AP (ITPK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 10568-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10568-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10568-1-AP (ITPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, mouse eye tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10568-1-AP targets ITPK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITPK1 fusion protein Ag0834
• Full Name: inositol 1,3,4-triphosphate 5/6 kinase
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC007428
• Gene Symbol: ITPK1
• Gene ID (NCBI): 3705
• RRID: AB_2129832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.