ITLN2 Polyclonal antibody

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  WB analysis using 18815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18815-1-AP (ITLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 18815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18815-1-AP (ITLN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITLN2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18815-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Intelectin-2, intelectin 2, HL2, HL 2, Endothelial lectin HL-2

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, mouse colon tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18815-1-AP targets ITLN2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: intelectin 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC143341
• Gene Symbol: ITLN2
• Gene ID (NCBI): 142683
• RRID: AB_3669302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWU7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITLN2 also named as UNQ2789 and PRO7179, may play a role in the defense system against pathogens. Intelectins (intestinal lectins) are highly conserved across chordate evolution and have been implicated in various human diseases, including Crohn's disease (CD). The human genome encodes two intelectin genes, intelectin-1 (ITLN1) and intelectin-2 (ITLN2). It is reproted that human ITLN2 is expressed in Paneth cells, where it localizes to secretory granules, and establish that ITLN2 is a highly abundant multimeric protein secreted into the lumen of the small intestine (PMID: 35182405). The calculated molecular weight of ITLN2 is 36 kDa.