ITK Polyclonal antibody

ITK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21525-1-AP

Synonyms

Kinase EMT, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, IL2 inducible T cell kinase, EMT

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Validation Data Gallery

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  WB analysis using 21525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21525-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21525-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21525-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 21525-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (21525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse spleen tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

21525-1-AP targets ITK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITK fusion protein Ag16132
• Full Name: IL2-inducible T-cell kinase
• Calculated Molecular Weight: 620 aa, 72 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC109077
• Gene Symbol: ITK
• Gene ID (NCBI): 3702
• RRID: AB_10733884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Immunol; Role of Signal-Transducing Adaptor Protein-1 for T Cell Activation and Pathogenesis of Autoimmune Demyelination and Airway Inflammation; Authors - Kota Kagohashi
human	WB	Cytotherapy; Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma; Authors - Wenjing Luo