ITGBL1 Polyclonal antibody

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  WB analysis using 30010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30010-1-AP (ITGBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ITGBL1 Polyclonal Antibody for WB, ELISA

Cat No. 30010-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Integrin beta like protein 1, ITGBL1, OSCP, TIED

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Tested Applications

• Positive WB detected in: HeLa cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30010-1-AP targets ITGBL1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITGBL1 fusion protein Ag31989
• Full Name: integrin, beta-like 1 (with EGF-like repeat domains)
• Calculated Molecular Weight: 494 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC036788
• Gene Symbol: ITGBL1
• Gene ID (NCBI): 9358
• RRID: AB_3086208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95965
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-like protein 1 (ITGBL1), also named as TIED or OSCP, is a secreted protein that contains ten tandem EGF-like repeats strikingly similar to those found in the cysteine-rich "stalk-like" structure of integrin beta subunits (PMID: 10051402). ITGBL1 participates in the regulation of fibrogenesis via interactions with transforming growth factor beta 1 (PMID: 28262670). ITGBL1 is also involved in the development and metastasis of many tumors (PMID: 32211321; 29772438; 26060017; 31190876).