ITGB1BP1 Polyclonal antibody

ITGB1BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12300-1-AP

Synonyms

Integrin cytoplasmic domain-associated protein 1, Integrin beta-1-binding protein 1, ICAP-1, ICAP1, ICAP 1

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Validation Data Gallery

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  WB analysis of PC-3 using 12300-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12300-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12300-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 12300-1-AP

  IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human heart using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 12300-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 12300-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse thymus tissue, human brain tissue
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: human heart tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITGB1BP1 fusion protein Ag2945
• Full Name: integrin beta 1 binding protein 1
• Calculated Molecular Weight: 200 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC012264
• Gene Symbol: ITGB1BP1
• Gene ID (NCBI): 9270
• RRID: AB_10640896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.