PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12300-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12300-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse thymus using 12300-1-AP
IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.
Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of PC-3 using 12300-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PC-3 cells, mouse thymus tissue, human brain tissue
Positive IP detected in
mouse thymus tissue
Positive IHC detected in
human heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
PC-3 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
12300-1-AP targets ITGB1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ITGB1BP1, also named as ICAP1, is a key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail. It plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. ITGB1BP1 promotes transcriptional activity of the MYC promoter.
PC-3 cells were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12300-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12300-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 12300-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 12300-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12300-1-AP (ITGB1BP1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 12300-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 12300-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 12300-1-AP (ITGB1BP1 antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse thymus using 12300-1-AP
IP result of anti-ITGB1BP1 (IP:12300-1-AP, 4ug; Detection:12300-1-AP 1:300) with mouse thymus tissue lysate 2800ug.
IF/ICC Figures
IF Staining of PC-3 using 12300-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ITGB1BP1 antibody (12300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
ITGB1BP1 Polyclonal antibody
Catalog Number
12300-1-AP
Citations
-
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
