Integrin Alpha 5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Alpha 5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 27224-1-AP

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

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Validation Data Gallery

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  WB analysis using 27224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27224-1-AP

  WB result of Integrin alpha-5 antibody (27224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

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  WB analysis of HeLa using 27224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27224-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (ITGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27224-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 2600 ug.

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  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 4000 ug.

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  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human placenta tissue, U2OS cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 27224-1-AP is Integrin Alpha 5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha 5 fusion protein Ag26098
• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 135-150 kDa
• GenBank Accession Number: NM_002205
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• RRID: AB_2880807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Clin Transl Hepatol; A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes; Authors - Beiyuan Hu
human	WB	Mol Cancer; ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner; Authors - Chuanpeng Zhang
human	WB	Biomater Res; Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment; Authors - Pengcheng Zhou ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

By WB, it labels a band at the expected molecular weight, but this band is very likely not the real protein, because it is increased during adipocyte differentiation, which should be decreased during the process according to literature.By IF, PFA fixation, it labels the protein in the nucleus. But according to literature, it is localised in the cell surface and the Gogli.

• Applications: Western Blot, Immunofluorescence,