ISLR Recombinant antibody

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  WB analysis of mouse retina using 83782-5-RR

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83782-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83782-5-RR (ISLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 83782-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83782-5-RR (ISLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83782-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83782-5-RR against Human ISLR were performed. The affinity constant is 0.369 nM.

ISLR Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83782-5-RR

Clone No.240727D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

UNQ189/PRO215, Immunoglobulin superfamily containing leucine-rich repeat protein, HsT17563, 240727D4

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Tested Applications

• Positive WB detected in: mouse retina tissue, NIH/3T3 cells
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83782-5-RR targets ISLR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ISLR fusion protein Ag19446
• Full Name: immunoglobulin superfamily containing leucine-rich repeat
• Calculated Molecular Weight: 428 aa, 46 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC022478
• Gene Symbol: ISLR
• Gene ID (NCBI): 3671
• RRID: AB_3671374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O14498
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)