IRG1 Polyclonal antibody

IRG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28436-1-AP

Synonyms

CAD, ACOD1

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Validation Data Gallery

• 

  WB analysis using 28436-1-AP

  IFN gamma+LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28436-1-AP (IRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28436-1-AP

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28436-1-AP (IRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: IFN gamma+LPS treated THP-1 cells, LPS treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

28436-1-AP targets IRG1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRG1 fusion protein Ag28913
• Full Name: immunoresponsive 1 homolog (mouse)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_001258406
• Gene Symbol: IRG1
• Gene ID (NCBI): 730249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654)

Publications

Species	Application	Title
mouse	WB	Apoptosis; IRG1/Itaconate inhibits proliferation and promotes apoptosis of CD69+CD103+CD8+ tissue-resident memory T cells in autoimmune hepatitis by regulating the JAK3/STAT3/P53 signalling pathway; Authors - Pei Zhou
mouse	WB	Int Immunopharmacol; IRG1/itaconate alleviates acute liver injury in septic mice by suppressing NLRP3 expression and its mediated macrophage pyroptosis via regulation of the Nrf2 pathway; Authors - Pei Zhou ; KD Validated
human	WB	J Inflamm Res; IRF2 Affects LPS- and IFN-γ-Induced Pro-Inflammatory Responses, Cell Viability, Migration and Apoptosis of Macrophages by Regulating IRG1; Authors - Ru-Xue Qin