IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A4A10

Cat no : 66835-1-Ig

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

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Validation Data Gallery

• 

  WB analysis of A549 using 66835-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66835-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66835-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66835-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, THP-1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66835-1-Ig targets IRF5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IRF5 fusion protein Ag5442
• Full Name: interferon regulatory factor 5
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC004201
• Gene Symbol: IRF5
• Gene ID (NCBI): 3663
• RRID: AB_2882178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.