IRF3 Recombinant antibody, PBS Only
IRF3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, zebrafish
Applications
WB, IHC, IF/ICC, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
2H6
Cat no : 80519-1-PBS
Synonyms
Validation Data Gallery
Product Information
80519-1-PBS targets IRF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, zebrafish samples.
Tested Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | interferon regulatory factor 3 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC009395 |
Gene Symbol | IRF3 |
Gene ID (NCBI) | 3661 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.