IRAK3 Recombinant antibody

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  WB analysis of THP-1 using 83876-2-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 83876-2-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83876-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 83876-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

IRAK3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83876-2-RR

Clone No.240975C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells, mouse lung tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83876-2-RR targets IRAK3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRAK3 fusion protein Ag4959
• Full Name: interleukin-1 receptor-associated kinase 3
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC057800
• Gene Symbol: IRAK3
• Gene ID (NCBI): 11213
• RRID: AB_3671461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y616
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).