IRAK1 Recombinant antibody

• 

  WB analysis using 86068-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 86068-3-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86068-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86068-3-RR against Human IRAK1 were performed. The affinity constant is 2.50 nM.

IRAK1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86068-3-RR

Clone No.250691C5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

EC:2.7.11.1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK 1, IRAK-1

---

Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86068-3-RR targets IRAK1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRAK1 fusion protein Ag0728
• Full Name: interleukin-1 receptor-associated kinase 1
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC014963
• Gene Symbol: IRAK1
• Gene ID (NCBI): 3654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51617
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)