IQUB Polyclonal antibody

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  IP experiment of mouse testis using 25605-1-AP

  IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse testis tissue lysate 1120 ug.

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  IP experiment of mouse spleen using 25605-1-AP

  IP result of anti-IQUB (IP:25605-1-AP, 4ug; Detection:25605-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

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  IHC staining of mouse testis using 25605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25605-1-AP (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IQUB Polyclonal Antibody for IHC, IP, ELISA

Cat No. 25605-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Synonyms

IQ motif and ubiquitin-like domain-containing protein

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Tested Applications

• Positive IP detected in: mouse spleen tissue, mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25605-1-AP targets IQUB in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IQUB fusion protein Ag19507
• Full Name: IQ motif and ubiquitin domain containing
• Calculated Molecular Weight: 791 aa, 93 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC128181
• Gene Symbol: IQUB
• Gene ID (NCBI): 154865
• RRID: AB_3085807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NA54
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).